LOCUS       40924_13980        6787 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCXGFP-1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Suemizu H.
  TITLE     Expression vector pCXGFP-1 DNA, complete sequence
  JOURNAL   Published Only in Database (2006)
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Suemizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2006) Contact:Hiroshi Suemizu Central Institute 
            for Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE   3  (bases 1 to 6787)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6787)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2006)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2006) Contact:Hiroshi Suemizu Central Institute for 
            Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..142
                     /label=polyoma virus enhancer
                     /note="polyoma virus enhancer"
                     /regulatory_class="enhancer"
     regulatory      149..281
                     /label=HSV-TK promoter
                     /note="HSV-TK promoter"
                     /regulatory_class="promoter"
     CDS             286..1086
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    1096..1144
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1151..1534
                     /label=CMV IE enhancer
                     /note="CMV IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        1154..1533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1536..1811
                     /label=chicken beta-actin promoter
     intron          1812..2820
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     exon            2821..2874
                     /note="rabbit beta-globin 3rd exon"
     misc_feature    2875..2880
                     /label=cloning site EcoRV
                     /note="cloning site EcoRV"
     CDS             2881..3006
                     /codon_start=1
                     /gene="MICA"
                     /product="truncated MHC class I chain related gene A
                     protein"
                     /label=MICA
                     /note="transmembrane domain; truncated MICA protein"
                     /protein_id="BAF36706.1"
                     /translation="QSHWQTFHVSAVAAAAAAIFVIIIFYVRCCKKKTSGDPPVAT"
     gene            2881..3006
                     /gene="MICA"
                     /label=MICA
     CDS             3007..3723
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    3876..3931
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4290..4306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4314..4330)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4338..4368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4383..4404)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4462..4658
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    4664..4798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5037..5625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5799..6656)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6657..6761)
                     /label=AmpR promoter
ORIGIN
        1 ggtcgagcag tgtggttttc aagaggaagc aaaaagcctc tccacccagg cctggaatgt
       61 ttccacccaa tgtcgagcag tgtggttttg caagaggaag caaaaagcct ctccacccag
      121 gcctggaatg tttccaccca atgtcgagca aaccccgccc agcgtcttgt cattggcgaa
      181 ttaattcgaa cacgcagatg cagtcggggc ggcgcggtcc caggtccact tcgcatatta
      241 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag ccaatatggg atcggccatt
      301 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      361 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      421 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      481 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      541 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      601 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      661 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      721 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      781 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat
      841 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
      901 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
      961 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1021 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1081 ttcttctgag gggatcggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     1141 gttcggatcc gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
     1201 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     1261 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1321 acgccaatag ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac
     1381 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     1441 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     1501 tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt
     1561 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     1621 attttgtgca gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg
     1681 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     1741 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     1801 cgcggcgggc gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc
     1861 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
     1921 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc
     1981 tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg
     2041 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct
     2101 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc
     2161 ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     2221 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc
     2281 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc
     2341 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     2401 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc
     2461 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     2521 agggcgcagg gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc
     2581 gcaccccctc tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     2641 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg
     2701 ccgcaggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     2761 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     2821 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcgatatc
     2881 cagagtcatt ggcagacatt ccatgtttct gctgttgctg ctgctgctgc tgctattttt
     2941 gttattatta ttttctatgt ccgttgttgt aagaagaaaa catcagggga tccaccggtc
     3001 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3061 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3721 aagtaaagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     3781 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     3841 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     3901 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     3961 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct
     4021 gccatgaaca aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     4081 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     4141 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     4201 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg
     4261 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4321 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4381 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4441 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     4501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4621 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     4681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     4801 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4861 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4921 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4981 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5101 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5161 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5221 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5281 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5341 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5401 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5461 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5521 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5581 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5641 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5701 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5761 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5821 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5881 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5941 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6001 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6061 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6121 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6181 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6241 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6301 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6361 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6421 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6481 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6541 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6601 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6661 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6721 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6781 ccacctg
//
